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Pretzels Without Salt are Called Baldies.The Average Pennsylvanian Eats Over 12-Pounds Each Year.The Average American Eats 2-Pounds of Pretzels Each Year.The Largest Pretzel on Record is 26.8-Feet Long and Weighed Over 840 Pounds.$550 Million Dollars of Pretzels Are Sold in The United States Annually.It has since been celebrated by pretzel enthusiasts all over the United States and even the world. This machine enabled bakeries to make almost 250 pretzels a minute.īecause of the popularity of pretzels in Pennsylvania, National Pretzel Day was established by a declaration by Pennsylvania Governor Ed Rendell in 2003. However, automation would eventually hit the market when the the first automated pretzel machine was introduced in 1935 by the Reading Pretzel Machinery Company. These workers are reputed to have hand-twisted up to 40 pretzels every single minute. Until the 1930s, pretzels were handmade by American workers. ![]() Even today, more than three-fourths of all pretzels made in the United States are made in Pennsylvania. And Pennsylvania established itself as the main producer of pretzels in North America. Included in these bread products were pretzels, which became a popular American treat. The German immigrants became known as Pennsylvanian Dutch and many of them began to open bakeries all over Pennsylvania to sell bread products. While no one really knows if this is true or not, one thing that is known is that pretzels became a popular food for Lent.ĭuring the 18th century, pretzels traveled with Germany immigrants as they immigrated to the United States. Some people say that the pretzel was invented to be used as a food for Lent and the peculiar shape of pretzels represents either people folding their hands in pray or the three knots of the Holy Trinity. They were such a prominent symbol of good luck, probably because they were associated with Christianity.īy the 1500s, it became common for people to eat pretzels in Germany on Good Friday. So much so, in fact, that German children often hung pretzels around their necks to celebrate New Years and pretzels were used to decorate Christmas trees. During this time, the pretzel was incorporated into the crests of German baker’s guilds in 1111.Įventually, pretzels would become known as a symbol of good luck. In order to obtain the first recorded evidence of the pretzel, you have to travel to 12th century Germany. However, this version of the pretzel origin is currently unconfirmed and there really is no evidence to support it. Supposedly, this is when monks baked little pieces of dough to give out as rewards to children who memorized their prayers. Which, according to myth, can be traced back to 7th century Italy. In order to full appreciate Pretzel Day, you first have to dig down to the origins of the pretzel. On this day, people enjoy pretzels in all their forms and discuss which variety is better, hard or soft pretzels. It’s a day in which people celebrate the pretzel – a snack that has been enjoyed since the Middle Ages. 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Notre Dame often can't touch the prospects that go to Duke or Carolina or Kansas or UCLA or even Virginia. I can't tell you that "none of the teams that do compete every year don't have some standards," but I will. The admission standard remains the standard. Now? Maybe one or two top 100 would be seriously interested in Notre Dame. Of those 10, maybe two, sometimes three would actually have a passing interest in the program. One former Irish assistant coach said years ago - a decade or so back - that even back then, in the mid-2000s, out of the top 100 high school seniors maybe 10 - maybe - would have the grades to get into Notre Dame for basketball. ![]() Tom Noie: Mike: Two questions in and and we've already reached the non-believer section. You can not tell me that none of the teams that do compete every year don't have some standards also. Maybe not get the one or two to make you a national champ every year but good enough to be in the top twenty and at least compete for the top half in the ACC. Just can not believe there are not three or four kids in the whole USA that are not smart enough to go to N.D. Roll through the 23 memories of Brey's 23 seasons for evidence. ![]() This isn't ending the way anyone imagined, but it was one hell of a ride. How many fans of a program that was dying a slow death would've taken nearly 500 wins? Three Sweet 16s? Two Elite Eights? A freaking ACC tournament championship after beating Duke and North Carolina? How many would've taken that? How about everybody. But rewind back to July 2000 when Mike Brey walked in the door of the Joyce Center. Should it have been better in the NCAA tournament? Absolutely. Tom Noie: Skill and leadership and, oh, by the way, a heck of a lot of memories and games and wins that nobody thought possible back in 2000. I thought his article Sunday highlighting the top 23 games under Coach Brey was particularly effective to remind us all of the skill & leadership Coach has given the players & fans over his 23 seasons. Definitely a boys clubħ) Customers can be super rude, which is compounded by the fact that they all are most likely carrying a gun or going to go shoot.ĩ) CEO is a penny pincher, don't expect anything but the bare minimum in terms of safety equipment or store supplies/maintenanceġ0) Can be easily fired if mistakes on federal paperwork are made (seen more people fired than I can countġ1) Most stores are understaffed, so expect to be doing the work of 2 employees.Guest: I congratulate Tom for his fair & balanced critique of ND b-ball over these past years. If you screw up or management doesn't like you, they will put you in the range for longer than the advertised "2 hrs per day"ĥ) Will only work you way up if management likes you. 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Hence, there is an unmet need for an integrative approach to simultaneously identify clonal population structure and the associated clonal genotypes from bulk DNA- and scRNA-seq data towards identifying intra-tumor heterogeneity in clonal gene expression profiles. However, the two-step approach does not fully utilize the available data as information in the scRNA data cannot be used to improve clonal population structure. Recent methods that seek to assign gene expression profiles to clones have treated the problem as a two-step procedure whereby the clonal population structure is identified and then scRNA data is aligned to clonal genotypes 17, 18. The increasing availability of single-cell RNA sequencing (scRNA-seq) data provides an approach to partially address this problem. Though the aforementioned methods can resolve clonal population structure, they cannot identify functional differences that result from the genomic heterogeneity. Recent advances in single-cell DNA sequencing (scDNA-seq) technologies have prompted the development of approaches better tailored to these data types 12, 13, 14 as well as methods that integrate scDNA-seq data with the bulk data for joint analysis for improved accuracy 15, 16. ![]() The limitations of clonal analysis using only the bulk method is well documented in the literature (e.g., refs. Early approaches used bulk sequence data coupled with computational deconvolution to address the admixed nature of bulk data 5, 6, 7, 8, 9. Inferring clonal population structure, genotypes, and trees from sequence data has been an active area of research in the past decade with implications for cancer treatment 2, 3, 4. The evolutionary relationship between clones can be represented by a phylogenetic tree or clonal tree. Though each cell is fundamentally distinct in cancer, there typically exist groups of cells that are genomically nearly identical, so-called clonal populations 1. We analyze high-grade serous ovarian cancer and breast cancer data to show that PhylEx exploits clonal expression profiles beyond what is possible with expression-based clustering methods and clear the way for accurate inference of clonal trees and robust phylo-phenotypic analysis of cancer.Ĭancer is an evolutionary process with ongoing mutational processes coupled with selection and drift leading to genetic diversity within the tumor cell populations. PhylEx outperforms the state-of-the-art methods both when comparing capacity for clonal tree reconstruction and for identifying clones. We evaluate PhylEx on synthetic and well-characterized high-grade serous ovarian cancer cell line datasets. ![]() We present PhylEx that integrates bulk genomics data with co-occurrences of mutations from single-cell RNA sequencing data to reconstruct high-fidelity clonal trees. 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